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lamp reactions  (New England Biolabs)


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    Structured Review

    New England Biolabs lamp reactions
    Lamp Reactions, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 367 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lamp+reaction/WarmStart+LAMP+Kit/10__3347_slash_phd__25083-71-6-17
    Average 97 stars, based on 367 article reviews
    lamp reactions - by Bioz Stars, 2026-09
    97/100 stars

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    Amplification:

    Article Title: The abundance of snail hosts mediates the effects of antagonist interactions between trematodes on the transmission of human schistosomes.
    Article Snippet: .. Each LAMP reaction contained an Isothermal Amplification Buffer II reaction buffer [20 mmol/L Tris-HCl, 10 mmol/L (NH4)2SO4, 150 mmol/L KCl, 2 mmol/L MgSO4, 0.1% Tween 20, pH 8.8 at 25 °C (New England Biolabs, UK)] at 1 X, additional MgSO4 at 3 mmol/L, dNTP at 1.0 mmol/L, internal primers FIP and BIP at 1.2 μmol/L, external primers F3 and B3 at 0.2 μmol/L, LOOP primers LB and LF at 0.4 μmol/L, 1 U of Bst 2.0 WarmStart DNA polymerase (New England Biolabs, UK), 1 μl of DNA sample, and ultrapure water for a total reaction volume of 10 μl as described in Blin et al., 2023 [24]. ..

    Article Title: A novel extraction-free dual HiFi-LAMP assay for detection of methicillin-sensitive and methicillin-resistant Staphylococcus aureus
    Article Snippet: We synthesized all these primers (by Shanghai Sangon Biotechnology Co., LTD) and performed comparative experiments to select the optimal LAMP primer sets for detection of MSSA and MRSA as previously described ( ). .. Each set of primers was performed in a 25 μL LAMP reaction containing 1 × isothermal amplification buffer, 8 mM MgSO4, 1.8 mM dNTP, 8 U Bst 4.0 DNA polymerase (Haigene, China), 0.15 U high-fidelity DNA polymerase (New England Biolabs, Inc., MA, USA), 0.4 mM SYTO9 (Life Technologies, CA, USA), 0.1 μM F3 and B3 each, 1.0 μM FIP and BIP each, and 0.6 μM LB and/or LF each. ..

    Article Title: The abundance of snail hosts mediates the effects of antagonist interactions between trematodes on the transmission of human schistosomes
    Article Snippet: .. Each LAMP reaction contained an Isothermal Amplification Buffer II reaction buffer [20 mmol/L Tris-HCl, 10 mmol/L (NH4) 2 SO 4 , 150 mmol/L KCl, 2 mmol/L MgSO 4 , 0.1% Tween 20, pH 8.8 at 25 °C (New England Biolabs, UK)] at 1 X, additional MgSO 4 at 3 mmol/L, dNTP at 1.0 mmol/L, internal primers FIP and BIP at 1.2 μmol/L, external primers F3 and B3 at 0.2 μmol/L, LOOP primers LB and LF at 0.4 μmol/L, 1 U of Bst 2.0 WarmStart DNA polymerase (New England Biolabs, UK), 1 μl of DNA sample, and ultrapure water for a total reaction volume of 10 μl as described in Blin et al., 2023 [ ]. ..

    Article Title: Development and utilization of a visual loop-mediated isothermal amplification coupled with a lateral flow dipstick (LAMP-LFD) assay for rapid detection of Echinostomatidae metacercaria in edible snail samples.
    Article Snippet: Trematodes belonging to the family Echinostomatidae are food-borne parasites which cause echinostomiasis in animals and humans.. This is a global public health issue, particularly in East and Southeast Asia.. A method to detect the infective stage of Echinostomatidae species is required to prevent transmission to humans.

    Article Title: A LEGO®-inspired pipette-free approach for a fully-integrated molecular diagnostic kit isothermally operated at near body temperature for the detection of antibacterial resistance.
    Article Snippet: Herein, we introduce a LEGO®-inspired molecular diagnostic microdevice fully integrated with DNA extraction, loop-mediated isothermal amplification (LAMP), and colorimetric detection functionalities for rapid detection of antibacterial resistance in a pipette-free manner.. The microdevice system is composed of a ready-to-use microdevice containing all necessary reagents and stamps that offer sample-to-answer diagnosis in a pipette-free manner.. In particular, antimicrobial resistance was analyzed through LAMP at a significantly reduced temperature of approximately 40 °C, combined with DNA extraction and detection, which were performed at room temperature (RT).



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